GolpHCat (TMEM87A), a unique voltage-dependent cation channel in Golgi apparatus, contributes to Golgi-pH maintenance and hippocampus-dependent memory

Hyunji Kang, Ah-reum Han, Aihua Zhang, Heejin Jeong, Wuhyun Koh, Jung Moo Lee, Hayeon Lee, Hee Young Jo, Miguel A. Maria-Solano, Mridula Bhalla, Jea Kwon, Woo Suk Roh, Jimin Yang, Hyun Joo An, Sun Choi (), Ho Min Kim () and C. Justin Lee ()
Additional contact information
Hyunji Kang: Life Science Cluster, Institute for Basic Science (IBS)
Ah-reum Han: Life Science Cluster, Institute for Basic Science (IBS)
Aihua Zhang: Ewha Womans University
Heejin Jeong: Chungnam National University
Wuhyun Koh: Life Science Cluster, Institute for Basic Science (IBS)
Jung Moo Lee: Life Science Cluster, Institute for Basic Science (IBS)
Hayeon Lee: Life Science Cluster, Institute for Basic Science (IBS)
Hee Young Jo: Chungnam National University
Miguel A. Maria-Solano: Ewha Womans University
Mridula Bhalla: Life Science Cluster, Institute for Basic Science (IBS)
Jea Kwon: Life Science Cluster, Institute for Basic Science (IBS)
Woo Suk Roh: Life Science Cluster, Institute for Basic Science (IBS)
Jimin Yang: Life Science Cluster, Institute for Basic Science (IBS)
Hyun Joo An: Chungnam National University
Sun Choi: Ewha Womans University
Ho Min Kim: Life Science Cluster, Institute for Basic Science (IBS)
C. Justin Lee: Life Science Cluster, Institute for Basic Science (IBS)

Nature Communications, 2024, vol. 15, issue 1, 1-24

Abstract: Abstract Impaired ion channels regulating Golgi pH lead to structural alterations in the Golgi apparatus, such as fragmentation, which is found, along with cognitive impairment, in Alzheimer’s disease. However, the causal relationship between altered Golgi structure and cognitive impairment remains elusive due to the lack of understanding of ion channels in the Golgi apparatus of brain cells. Here, we identify that a transmembrane protein TMEM87A, renamed Golgi-pH-regulating cation channel (GolpHCat), expressed in astrocytes and neurons that contributes to hippocampus-dependent memory. We find that GolpHCat displays unique voltage-dependent currents, which is potently inhibited by gluconate. Additionally, we gain structural insights into the ion conduction through GolpHCat at the molecular level by determining three high-resolution cryogenic-electron microscopy structures of human GolpHCat. GolpHCat-knockout mice show fragmented Golgi morphology and altered protein glycosylation and functions in the hippocampus, leading to impaired spatial memory. These findings suggest a molecular target for Golgi-related diseases and cognitive impairment.

Date: 2024
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DOI: 10.1038/s41467-024-49297-8

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