Bioelectrocatalytic carbon dioxide reduction by an engineered formate dehydrogenase from Thermoanaerobacter kivui

Weisong Liu, Kuncheng Zhang, Jiang Liu, Yuanming Wang, Meng Zhang, Huijuan Cui, Junsong Sun and Lingling Zhang ()
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Weisong Liu: Tianjin Airport Economic Area
Kuncheng Zhang: Tianjin Airport Economic Area
Jiang Liu: Chinese Academy of Sciences
Yuanming Wang: Chinese Academy of Sciences
Meng Zhang: Chinese Academy of Sciences
Huijuan Cui: Tianjin Airport Economic Area
Junsong Sun: University of Chinese Academy of Sciences
Lingling Zhang: Tianjin Airport Economic Area

Nature Communications, 2024, vol. 15, issue 1, 1-11

Abstract: Abstract Electrocatalytic carbon dioxide (CO2) reduction by CO2 reductases is a promising approach for biomanufacturing. Among all known biological or chemical catalysts, hydrogen-dependent carbon dioxide reductase from Thermoanaerobacter kivui (TkHDCR) possesses the highest activity toward CO2 reduction. Herein, we engineer TkHDCR to generate an electro-responsive carbon dioxide reductase considering the safety and convenience. To achieve this purpose, a recombinant Escherichia coli TkHDCR overexpression system is established. The formate dehydrogenase is obtained via subunit truncation and rational design, which enables direct electron transfer (DET)-type bioelectrocatalysis with a near-zero overpotential. By applying a constant voltage of −500 mV (vs. SHE) to a mediated electrolytic cell, 22.8 ± 1.6 mM formate is synthesized in 16 h with an average production rate of 7.1 ± 0.5 μmol h−1cm−2, a Faradaic efficiency of 98.9% and a half-cell energy efficiency of 94.4%. This study provides an enzyme candidate for high efficient CO2 reduction and opens up a way to develop paradigm for CO2-based bio-manufacturing.

Date: 2024
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DOI: 10.1038/s41467-024-53946-3

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