A self-eliminating allelic-drive reverses insecticide resistance in Drosophila leaving no transgene in the population

Ankush Auradkar, Rodrigo M. Corder, John M. Marshall and Ethan Bier ()
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Ankush Auradkar: University of California San Diego
Rodrigo M. Corder: University of São Paulo
John M. Marshall: University of California
Ethan Bier: University of California San Diego

Nature Communications, 2024, vol. 15, issue 1, 1-10

Abstract: Abstract Insecticide resistance (IR) poses a significant global challenge to public health and welfare. Here, we develop a locally-acting unitary self-eliminating allelic-drive system, inserted into the Drosophila melanogaster yellow (y) locus. The drive cassette encodes both Cas9 and a single gRNA to bias inheritance of the favored wild-type (1014 L) allele over the IR (1014 F) variant of the voltage-gated sodium ion channel (vgsc) target locus. When enduring a fitness cost, this transiently-acting drive can increase the frequency of the wild-type allele to 100%, depending on its seeding ratio, before being eliminated from the population. However, in a fitness-neutral “hover” mode, the drive maintains a constant frequency in the population, completely converting IR alleles to wild-type, even at low initial seeding ratios.

Date: 2024
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DOI: 10.1038/s41467-024-54210-4

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