Endothelial Pim3 kinase protects the vascular barrier during lung metastasis

Niina M. Santio, Keerthana Ganesh, Pihla P. Kaipainen, Aleksi Halme, Fatemeh Seyednasrollah, Emad Arbash, Satu Hänninen, Riikka Kivelä, Olli Carpen and Pipsa Saharinen ()
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Niina M. Santio: University of Helsinki
Keerthana Ganesh: University of Helsinki
Pihla P. Kaipainen: University of Helsinki
Aleksi Halme: University of Helsinki
Fatemeh Seyednasrollah: University of Helsinki
Emad Arbash: University of Helsinki
Satu Hänninen: Research Programs Unit University of Helsinki
Riikka Kivelä: Biomedicum Helsinki
Olli Carpen: Research Programs Unit University of Helsinki
Pipsa Saharinen: University of Helsinki

Nature Communications, 2024, vol. 15, issue 1, 1-18

Abstract: Abstract Endothelial cells (ECs) form a tissue-specific barrier for disseminating cancer cells in distant organs. However, the molecular regulation of the ECs in the metastatic niche remains unclear. Here, we analyze using scRNA-Seq, the transcriptional reprogramming of lung ECs six hours after the arrival of melanoma cells in mouse lungs. We discover a reactive capillary EC cluster (rCap) that increases from general capillary ECs in response to infiltrating cancer cells. rCap is enriched for angiogenic and inflammatory pathways and is also found in human lung datasets. The JAK-STAT activated oncogenic Pim3 kinase is a marker of rCap, being upregulated in spontaneous metastasis models. Notably, PIM inhibition increases vascular leakage and metastatic colonization and impairs the EC barrier by decreasing the junctional cadherin-5 and catenins α, β and δ. These results highlight the pulmonary endothelium’s plasticity and its protection by PIM3, which may impair the efficacy of PIM inhibitors in cancer therapies.

Date: 2024
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DOI: 10.1038/s41467-024-54445-1

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