Gastruloids are competent to specify both cardiac and skeletal muscle lineages

Laurent Argiro, Céline Chevalier, Caroline Choquet, Nitya Nandkishore, Adeline Ghata, Anaïs Baudot, Stéphane Zaffran () and Fabienne Lescroart ()
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Laurent Argiro: Marseille Medical Genetics (MMG)
Céline Chevalier: Marseille Medical Genetics (MMG)
Caroline Choquet: Marseille Medical Genetics (MMG)
Nitya Nandkishore: Marseille Medical Genetics (MMG)
Adeline Ghata: Marseille Medical Genetics (MMG)
Anaïs Baudot: Marseille Medical Genetics (MMG)
Stéphane Zaffran: Marseille Medical Genetics (MMG)
Fabienne Lescroart: Marseille Medical Genetics (MMG)

Nature Communications, 2024, vol. 15, issue 1, 1-19

Abstract: Abstract Cardiopharyngeal mesoderm contributes to the formation of the heart and head muscles. However, the mechanisms governing cardiopharyngeal mesoderm specification remain unclear. Here, we reproduce cardiopharyngeal mesoderm specification towards cardiac and skeletal muscle lineages with gastruloids from mouse embryonic stem cells. By conducting a comprehensive temporal analysis of cardiopharyngeal mesoderm development and differentiation in gastruloids compared to mouse embryos, we present the evidence for skeletal myogenesis in gastruloids. We identify different subpopulations of cardiomyocytes and skeletal muscles, the latter of which most likely correspond to different states of myogenesis with “head-like” and “trunk-like” skeletal myoblasts. In this work, we unveil the potential of gastruloids to undergo specification into both cardiac and skeletal muscle lineages, allowing the investigation of the mechanisms of cardiopharyngeal mesoderm differentiation in development and how this could be affected in congenital diseases.

Date: 2024
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DOI: 10.1038/s41467-024-54466-w

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