Quantitative determination of the spatial distribution of components in single cells with CellDetail

Tanja Schuster (), Amanda Amoah, Angelika Vollmer, Gina Marka, Julian Niemann, Mehmet Saçma, Vadim Sakk, Karin Soller, Mona Vogel, Ani Grigoryan, Meinhard Wlaschek, Karin Scharffetter-Kochanek, Medhanie Mulaw and Hartmut Geiger ()
Additional contact information
Tanja Schuster: Ulm University
Amanda Amoah: Ulm University
Angelika Vollmer: Ulm University
Gina Marka: Ulm University
Julian Niemann: Ulm University
Mehmet Saçma: Ulm University
Vadim Sakk: Ulm University
Karin Soller: Ulm University
Mona Vogel: Ulm University
Ani Grigoryan: Ulm University
Meinhard Wlaschek: Ulm University
Karin Scharffetter-Kochanek: Ulm University
Medhanie Mulaw: Ulm University
Hartmut Geiger: Ulm University

Nature Communications, 2024, vol. 15, issue 1, 1-13

Abstract: Abstract The distribution of biomolecules within cells changes upon aging and diseases. To quantitatively determine the spatial distribution of components inside cells, we built the user-friendly open-source 3D-cell-image analysis platform Cell Detection and Analysis of Intensity Lounge (CellDetail). The algorithm within CellDetail is based on the concept of the dipole moment. CellDetail provides quantitative values for the distribution of the polarity proteins Cdc42 and Tubulin in young and aged hematopoietic stem cells (HSCs). Septin proteins form networks within cells that are critical for cell compartmentalization. We uncover a reduced level of organization of the Septin network within aged HSCs and within senescent human fibroblasts. Changes in the Septin network structure might therefore be a common feature of aging. The level of organization of the network of Septin proteins in aged HSCs can be restored to a youthful level by pharmacological attenuation of the activity of the small RhoGTPase Cdc42.

Date: 2024
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DOI: 10.1038/s41467-024-54638-8

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