Subtilase SBT5.2 inactivates flagellin immunogenicity in the plant apoplast

Pierre Buscaill, Nattapong Sanguankiattichai, Farnusch Kaschani, Jie Huang, Brian C. Mooney, Yuge Li, Joy Lyu, Daniela Sueldo, Markus Kaiser and Renier A. L. van der Hoorn ()
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Pierre Buscaill: University of Oxford
Nattapong Sanguankiattichai: University of Oxford
Farnusch Kaschani: University of Duisburg-Essen
Jie Huang: University of Oxford
Brian C. Mooney: University of Oxford
Yuge Li: University of Oxford
Joy Lyu: University of Oxford
Daniela Sueldo: University of Oxford
Markus Kaiser: University of Duisburg-Essen
Renier A. L. van der Hoorn: University of Oxford

Nature Communications, 2024, vol. 15, issue 1, 1-13

Abstract: Abstract Most angiosperm plants recognise the 22-residue flagellin (flg22) epitope in bacterial flagellin via homologs of cell surface receptor FLS2 (flagellin sensitive-2) and mount pattern-triggered immune responses. However, flg22 is buried within the flagellin protein indicating that proteases might be required for flg22 release. Here, we demonstrate the extracellular subtilase SBT5.2 not only releases flg22, but also inactivates the immunogenicity of flagellin and flg22 by cleaving within the flg22 epitope, consistent with previous reports that flg22 is unstable in the apoplast. The prolonged lifetime of flg22 in sbt5.2 mutant plants results in increased bacterial immunity in priming assays, indicating that SBT5.2 counterbalances flagellin immunogenicity to provide spatial-temporal control and restrict costly immune responses and that bacteria take advantage of the host proteolytic machinery to avoid detection by flagellin having a protease-sensitive flg22 epitope.

Date: 2024
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DOI: 10.1038/s41467-024-54790-1

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