Microscopy Image Browser: A Platform for Segmentation and Analysis of Multidimensional Datasets

Ilya Belevich, Merja Joensuu, Darshan Kumar, Helena Vihinen and Eija Jokitalo

PLOS Biology, 2016, vol. 14, issue 1, 1-13

Abstract: Understanding the structure–function relationship of cells and organelles in their natural context requires multidimensional imaging. As techniques for multimodal 3-D imaging have become more accessible, effective processing, visualization, and analysis of large datasets are posing a bottleneck for the workflow. Here, we present a new software package for high-performance segmentation and image processing of multidimensional datasets that improves and facilitates the full utilization and quantitative analysis of acquired data, which is freely available from a dedicated website. The open-source environment enables modification and insertion of new plug-ins to customize the program for specific needs. We provide practical examples of program features used for processing, segmentation and analysis of light and electron microscopy datasets, and detailed tutorials to enable users to rapidly and thoroughly learn how to use the program.This Community Page describes a freely available, open-source software that implements and integrates a range of manual and semi-automated segmentation tools for processing and quantifying light and electron microscopy data.

Date: 2016
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pbio00:1002340

DOI: 10.1371/journal.pbio.1002340

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