MICROCLONAL PROPAGATION OF CRATAEGUS MONOGYNA JACQ. IN VITRO

Nana Zarnadze (), Ketevan Dolidze (), Sophiko Manjgaladze (), Nazi Turmanidze (), Jana Chitanava (), Gia Bolkvadze () and Eteri Jakeli ()
Additional contact information
Nana Zarnadze: Batumi Shota Rustaveli State University, Batumi
Ketevan Dolidze: Batumi Shota Rustaveli State University, Batumi
Sophiko Manjgaladze: Batumi Shota Rustaveli State University, Batumi
Nazi Turmanidze: Batumi Shota Rustaveli State University, Batumi
Jana Chitanava: Batumi Shota Rustaveli State University, Batumi
Gia Bolkvadze: Batumi Shota Rustaveli State University, Batumi
Eteri Jakeli: Batumi Shota Rustaveli State University, Batumi

CBU International Conference Proceedings, 2019, vol. 7, issue 0, 1020-1025

Abstract: We have researched Crataegus Monogina Jacq. microclonal propagation and in vitro mass regeneration processes. For this experiment we used the asleep buds isolated from maternal plants and in vitro cultivated apical and axillary buds as the effective explants. The high coefficient of micropropagation was provided with Gamborg Medium (B5) nutrient medium added alongside the hormones of cytokinin nature. The best result was achieved in the case of introduction of 2-Isopentenyladenine (2-iP) at concentration of 5-10 mcm and Benzilaminopurine (BAP) at concentration of 10-15 mcm. For stimulation of the mictropropagation process it is reasonable to add a small dose of the auxin hormone 1-Naphtylacetic acid (NAA) together with the cytokinins to the nutritive medium. It has been shown that the growth of micropropagation coefficient in pro rata the kinds and concentration of phytohormones. Regenerated microshoots had a good capacity of rooting. The most effective method for rooting purpose was the introduction of Indole- 3 –butyric acid (IBA) at concentration of 5mcm into 1 /2 B5 nutrient medium. The rooted regenerant plants successfully adopted in vivo.

Keywords: In Vitro Culture; Proliferation; Plant regeneration; Micropropagation; Phytohormones; Adventive buds (search for similar items in EconPapers)
Date: 2019
References: Add references at CitEc
Citations:

Downloads: (external link)
https://ojs.journals.cz/index.php/CBUIC/article/view/1494/2024 (application/pdf)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:aad:iseicj:v:7:y:2019:i:0:p:1020-1025

DOI: 10.12955/cbup.v7.1494

Access Statistics for this article

More articles in CBU International Conference Proceedings from ISE Research Institute
Bibliographic data for series maintained by Petr Hájek ().