Electrical Bioimpedance Spectroscopy as Biosensor Technique to Identify Pluripotent Stem Cells and Fibroblasts

Vazquez-Zapien Gj, Mata-Miranda Mm, Guerrero-Robles Ci, Sanchez-Monroy V, Lozano-Trenado Lm and Gonzalez-Diaz Ca
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Vazquez-Zapien Gj: Escuela Militar de Medicina, Centro Militar de Ciencias de la Salud, SEDENA, México
Mata-Miranda Mm: Escuela Superior de Medicina, Instituto Politécnico Nacional, México
Sanchez-Monroy V: Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, México
Gonzalez-Diaz Ca: Escuela Militar de Graduados de Sanidad, Centro Militar de Ciencias de la Salud, SEDENA, México

International Journal of Cell Science & Molecular Biology, 2017, vol. 3, issue 2, 30-35

Abstract: Pluripotent stem cells have the capacity to differentiate into specialized cells, and it has relevance in the engineering of tissue regeneration. The identification and characterization of diverse cells types requires complex techniques as flow cytometry, immunocytochemistry and the exploration of molecular markers; such techniques require infrastructure and qualified personnel. The objective of this study was to analyse the use of Electrical Bioimpedance Spectroscopy (EBIS) measurements as non-complex alternative technique to identify populations of undifferentiated pluripotent mouse Embryonic Stem Cells (mESCs) and Mouse Embryonic Fibroblasts (MEFs). EBIS measurements were compared in populations of pluripotent stem cells and fibroblasts which were characterized previously using Fourier Transform Infrared (FTIR) spectroscopy and fluorescence microscopy. The results indicate that EBIS technique has potential sensitivity at certain frequency range to discriminate between both evaluated cell populations. Additional studies with different concentrations in order to evaluate quantitatively the sensitivity and specificity of the proposed technique are recommended.

Keywords: juniper publishers; Journal of Cell Science; Molecular Biology; International Journal of Cell Science; International Journal of Cell Science & Molecular Biology; molecular biology journals list; molecular biology impact factor; molecular biology scholarly journal; molecular biology online journals; molecular biology research papers; scientific journals; juniper publishers review (search for similar items in EconPapers)
Date: 2017
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Persistent link: https://EconPapers.repec.org/RePEc:adp:ijcsmb:v:3:y:2017:i:2:p:30-35

DOI: 10.19080/IJCSMB.2017.03.555610

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