Isolation and Phenotypic Detection of Metallo- Beta-Lactamase (MBL)-Producing Klebsiella Species from Cow Anal Swabs

Ejikeugwu Chika, Oguejiofor Benigna, Ogene Lilian, Iroha Ifeanyichukwu, Duru Carissa, Eluu Stanley and Ezeador Chika
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Iroha Ifeanyichukwu: Department of Applied Microbiology, Ebonyi State University, Abakaliki
Duru Carissa: Department of Pharmaceutical Microbiology and Biotechnology, Nnamdi Azikiwe University, Awka, NIgeria
Eluu Stanley: Department of Biological Sciences (Biotechnology Programme), Ebonyi State University, Abakaliki
Ezeador Chika: Department of Microbiology and Parasitology, Nnamdi Azikiwe University Teaching Hospital (NAUTH), Nnewi, Nigeria

Global Journal of Pharmacy & Pharmaceutical Sciences, 2017, vol. 2, issue 3, 54-58

Abstract: The production of β-lactamase enzymes such as metallo β-lactamases (MBLs) is one of the most distributed resistance mechanisms engineered by Gram-negative bacteria towards β-lactam antibiotics. MBLs confer on bacteria the ability to hydrolyze and become resistant to the carbapenems such as imipenem. This study was aimed at isolating and detecting the production of MBLs from Klebsiella species that emanated from anal swabs of cow in a local abattoir. A total of 31 anal swab samples were bacteriologically analyzed for the isolation of Klebsiella species which were identified using standard microbiological techniques. Antimicrobial susceptibility testing was carried out using the modified Kirby-Bauer disk diffusion method on Mueller-Hinton agar plates. The isolated Klebsiella species was screened for MBL production by determining their susceptibility to the carbapenems and this was confirmed using the EDTA-inhibition-based assay for phenotypic detection of MBLs. A total of 18 Klebsiella species was isolated from the cow anal swab samples

Keywords: juniper publishers:Journal of Pharmacy; Global Journal of Pharmacy; Pharmaceutical Sciences; Pharmacy & Pharmaceutical Sciences; pharmaceutical sciences journals; omics online; open access; drug discovery; Clinical Trials; juniper publishers open access journals; juniper publishers reivew (search for similar items in EconPapers)
Date: 2017
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Persistent link: https://EconPapers.repec.org/RePEc:adp:jgjpps:v:2:y:2017:i:3:p:54-58

DOI: 10.19080/GJPPS.2017.02.555586

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