PRELIMINARY STUDIES TOWARDS THE PRODUCTION OF THE INSECTICIDE ROTENONE USING CELL CULTURE

G. Sirju-Charran, A. Maxwell and N. Lackhan

No 259099, 27th Annual Meeting, July 14-20, 1991, Roseau, Dominica from Caribbean Food Crops Society

Abstract: Detection and quantification of rotenone in the different organs of the plant Pachyrhizus erosus (1) Urban, was achieved by High Pressure Liquid Chromatography (HPLC) using a reverse-phase column. The seeds were found to contain 0,15% rotenone on a dry weight basis, while the tubers, leaves, and the pod shells contained negligible amounts. In attempting to induce high rotenone production in vitro, leaf discs and cotyledon sections of P. erosus were used as explant and these placed on a supplement Murashige and Skoog medium. Culture, are being grown under varying condition, of light requirement, sugar source, and hormone requirement in order to determine the optimum conditions for rotenone production.

Keywords: Crop Production/Industries; Research and Development/Tech Change/Emerging Technologies (search for similar items in EconPapers)
Pages: 4
Date: 1991-07-14
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Persistent link: https://EconPapers.repec.org/RePEc:ags:cfcs91:259099

DOI: 10.22004/ag.econ.259099

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