Molecular Detection of Beta-Lactam Resistance Genes in Staphylococcus Aureus Isolated From Women in Nasarawa State, Nigeria

Joseph Wg, Oti Bv*, Tsaku Ap, Ajegena Sa and Ajegena Bk
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Joseph Wg: Department of Microbiology, Nasarawa State University, P. M. B. 1022, Keffi, Nigeria
Oti Bv*: Department of Microbiology, Nasarawa State University, P. M. B. 1022, Keffi, Nigeria
Tsaku Ap: Department of Microbiology, Nasarawa State University, P. M. B. 1022, Keffi, Nigeria
Ajegena Sa: Department of Microbiology, Nasarawa State University, P. M. B. 1022, Keffi, Nigeria
Ajegena Bk: Department of Public Health, Anglia Ruskin University, Cambridge Campus, UK

International Journal of Healthcare and Medical Sciences, 2018, vol. 4, issue 5, 60-65

Abstract: Resistance to antimicrobials by pathogenic microorganisms has raised serious global clinical concerns in recent times. The present study aimed at detection of ?-lactam resistance genes in S. aureus isolates from women with symptomatic and asymptomatic cases of urinary tract infections in Nasarawa state, Nigeria. A total of 200 non-repetitive midstream urinal samples were analysed and 50 (29%) bacterial isolates were identified as S. aureus. The susceptibility profile of the bacterial isolates to tested antibiotics was Nitrofurantoin (74.1%), Gentamicin (72.4%), Ciprofloxacin (65.5%), Ofloxacin (56.9), Augmentin (36.2%), Cotrimozazole (29.3%), Ampicillin (27.6%), Erythromycin (25.8%), Ceftazidine (20.7%) and Cefurozime (10.3%). Thirteen bacterial isolates were found to be resistant to all ?-lactam antibiotics tested, out of which 7 were confirmed ?-lactamase producers using the acidometric and iodometric methods. The detection of ?-lactamase genes (blaZ, blaI and blaR1) was carried out and only five of the isolates were found to be expressing the blaI genes. This research finding suggests that ?-lactam resistance by S. aureus may not be dependent only on the blaZ, blaI and blaR1 genes.

Keywords: Staphylococcus aureus; ?-lactamase; Urinary tract infection; Antibiotic susceptibility. (search for similar items in EconPapers)
Date: 2018
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