Comparison of Three Methods of DNA Extraction from Parachipteria willmanni (Acari: Oribatida) Collected in Turkey

Sedat Per () and Fahriye Sümer Ercan
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Sedat Per: Department of Biology, Faculty of Arts and Science, Bozok University, 66900, Yozgat-Turkey
Fahriye Sümer Ercan: Department of Genetic and Bioengineering, Faculty of Architecture and Engineering, Ahi Evran University, 40200, Kırşehir-Turkey

Journal of Biotechnology Research, 2015, vol. 1, issue 4, 16-20

Abstract: We compared three DNA extraction methods; a Chelex resin (C100), Qiagen DNA extraction kit and Cethyl Trimethyl Ammonium Bromide (CTAB) protocol that obtained from Parachipteria willmanni van der Hammen, 1952 mite specimens preserved in ethanol. The aim of the study is to find the most efficient protocol for obtaining DNA from Parachipteria willmanni. All methods are successful in isolating DNA from P. willmanni, but among these three methods, maximum amount of DNA is obtained with Chelex-method. DNAs obtained by these three methods were successfully applied to RAPD-PCR.

Keywords: Chelex-method; DNA extraction; Oribatida; Parachipteria willmanni; RAPD-PCR. (search for similar items in EconPapers)
Date: 2015
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Persistent link: https://EconPapers.repec.org/RePEc:arp:rjbarp:2015:p:16-20

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