Exploring the Changes of Resistant Genes Expression in Groundnuts Arachis Hypogea In Response to Aspergillus Flavus Exposure at Seedling Stage

Robert O. Okayo, Darius O. Andika, Mathews M. Dida, George O. K’Otuto and Bernard M. Gichimu
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Robert O. Okayo: Department of Plant, Animal and Food Sciences, Jaramogi Oginga Odinga University of Science and Technology, Bondo, Kenya
Darius O. Andika: Department of Plant, Animal and Food Sciences, Jaramogi Oginga Odinga University of Science and Technology, Bondo, Kenya
Mathews M. Dida: Department of Applied Sciences, Maseno University, Maseno, Kenya
George O. K’Otuto: Department of Applied Sciences, Maseno University, Maseno, Kenya
Bernard M. Gichimu: Department of Agricultural Resource Management, University of Embu, Embu, Kenya

International Journal of Research and Scientific Innovation, 2020, vol. 7, issue 7, 203-208

Abstract: Aspergillus flavus infect groundnut seeds and produce secondary metabolites, aflatoxins. The aflatoxins are associated with various diseases in domestic animals and humans globally. Mitigating the aflatoxin contamination in crops through the development of cultivars tolerant to fungus colonization and aflatoxin contamination has been considered the most cost-effective measure. This research was conducted to ascertain that the resistance genes identified in the previous transcriptome analysis were involved in groundnut defense mechanisms to A. flavus infection. Eight genes were selected for additional scrutiny through the real time PCR on a groundnut seedling at an interval of 2 days within a 7-day period. The results indicate a network of gene expression patterns in a sequential order in both resistance and susceptible lines at a seedling stage. The peak expression level per gene indicates the time gene action was crucial. We conclude that these genes are involved in groundnut resistance to A. flavus infection and provide important targets for the molecular marker screening.

Date: 2020
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Persistent link: https://EconPapers.repec.org/RePEc:bjc:journl:v:7:y:2020:i:7:p:203-208

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