FT-IR spectroscopic characteristics of differently cultivated Escherichia coli

Zdenek Filip, Susanne Hermann and Kateřina Demnerová
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Zdenek Filip: Federal Environmental Agency, Langen Branch, Langen, Germany
Susanne Hermann: Federal Environmental Agency, Langen Branch, Langen, Germany
Kateřina Demnerová: Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic

Czech Journal of Food Sciences, 2008, vol. 26, issue 6, 458-463

Abstract: FT-IR spectra were recorded of Escherichia coli cell mass with the aim of obtaining spectral traits possibly useful in a rapid detection and characterisation of this indicator bacterium. A well differentiated spectrum was obtained from the cell mass harvested in a stationary phase of growth, e.g., after 24 h, from a minimum nutrient broth. The cell mass, harvested either earlier or grown in nutrient solutions which contained an enhanced carbon or nitrogen concentrations delivered somewhat different IR spectra, apparently due to a higher content of nucleic acid components as related to other structural constituents of bacterial cells. Consequently, the FT-IR spectra of E. coli, although rather rapidly to collect, seem only capable of delivering useful and reproducible information if the cell mass is obtained under standardised cultural conditions.

Keywords: Escherichia coli; FT-IR spectroscopy; cell mass traits (search for similar items in EconPapers)
Date: 2008
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:26:y:2008:i:6:id:14-2008-cjfs

DOI: 10.17221/14/2008-CJFS

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