Isolation, identification and antibiotic susceptibility of nis+ Lactococcus lactis from dairy and non-dairy sources

Priti Khemariya, Sudhir Singh, Gopal Nath and Anil K. Gulati
Additional contact information
Priti Khemariya: Post Harvest Technology Lab, Indian Institute of Vegetable Research, Jakkhini, Shahanshahpur, Varanasi (UP), India
Sudhir Singh: Post Harvest Technology Lab, Indian Institute of Vegetable Research, Jakkhini, Shahanshahpur, Varanasi (UP), India
Gopal Nath: Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi (UP), India
Anil K. Gulati: Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi (UP), India

Czech Journal of Food Sciences, 2013, vol. 31, issue 4, 323-331

Abstract: Eight isolates of Lactococcus lactis subsp. lactis were isolated and identified by phenotypic and molecular characterisation out of 23 isolates of lactic acid bacteria (LAB) from different dairy and non-dairy sources. Out of eight strains, four were obtained from dairy and four from non-dairy sources. All eight strains of L. lactis subsp. lactis were able to produce zones of inhibition against the Lactobacillus acidophilus NCDC 015. The antimicrobial agent produced by the isolates inhibited the growth of a range of related lactic acid bacteria and certain Gram positive food-borne microorganisms. The antimicrobial agent, i.e. nisin, produced by the strains was confirmed by PCR amplification of nisin gene sequences of 174 bp size. Antibiotic susceptibility test to 21 different types of antibiotics was evaluated. All the isolates were resistant to fosfomycin, cefepime, amikacin, kanamycin, neomycin, nalidixic acid, pipemidic acid, norfloxacin, sulphadiazine, colistin, polymixin, teicoplanin, nystatin, and amphotericin B but susceptible to ampicillin, erythromycin, spiramycin, spectinomycin, ciprofloxacin, rifampicin, and trimethoprim.

Keywords: Lactococcus lactis subsp. lactis; antimicrobial agent; nisin (search for similar items in EconPapers)
Date: 2013
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://cjfs.agriculturejournals.cz/doi/10.17221/316/2012-CJFS.html (text/html)
http://cjfs.agriculturejournals.cz/doi/10.17221/316/2012-CJFS.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:31:y:2013:i:4:id:316-2012-cjfs

DOI: 10.17221/316/2012-CJFS

Access Statistics for this article

Czech Journal of Food Sciences is currently edited by Ing. Zdeňka Náglová Ph.D.

More articles in Czech Journal of Food Sciences from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().