Sperm quality improvement of cryopreserved boar semen through colloidal centrifugation gradient

Francisco Sevilla, Ignacio Araya-Zúñiga, Patricia Cervantes-Acosta, Antonio Hernández-Beltrán, Manuel Barrientos, Belisario Domínguez and Anthony Valverde
Additional contact information
Francisco Sevilla: Doctorado en Ciencias Naturales para el Desarrollo (DOCINADE), Instituto Tecnológico de Costa Rica, Universidad Nacional, Universidad Estatal a Distancia, Alajuela, Costa Rica
Ignacio Araya-Zúñiga: Laboratory of Animal Reproduction, School of Agronomy, Research and Development Center for Sustainable Agriculture in the Humid Tropics, Costa Rica Institute of Technology, San Carlos Campus, Alajuela, Costa Rica
Patricia Cervantes-Acosta: School of Veterinary Medicine and Animal Husbandry, Master's in Animal Science Reproduction Biology, Biotechnology for Animal Health and Reproduction, Veracruz University, Veracruz, México
Antonio Hernández-Beltrán: School of Veterinary Medicine and Animal Husbandry, Master's in Animal Science Reproduction Biology, Biotechnology for Animal Health and Reproduction, Veracruz University, Veracruz, México
Manuel Barrientos: School of Veterinary Medicine and Animal Husbandry, Master's in Animal Science Reproduction Biology, Biotechnology for Animal Health and Reproduction, Veracruz University, Veracruz, México
Belisario Domínguez: School of Veterinary Medicine and Animal Husbandry, Master's in Animal Science Reproduction Biology, Biotechnology for Animal Health and Reproduction, Veracruz University, Veracruz, México
Anthony Valverde: Laboratory of Animal Reproduction, School of Agronomy, Research and Development Center for Sustainable Agriculture in the Humid Tropics, Costa Rica Institute of Technology, San Carlos Campus, Alajuela, Costa Rica

Czech Journal of Animal Science, 2025, vol. 70, issue 11, 489-497

Abstract: Boar semen cryopreservation is limited due to the lower thermal shock resistance of sperm cells. A colloidal centrifugation gradient is an enhancement to cryopreservation that could improve the frozen boar semen quality. Cryopreserved boar semen quality was evaluated using a commercial colloidal centrifugation gradient. A total of 15 ejaculates from 5 boars were evaluated across two treatments: a control without colloidal centrifugation and a treatment with commercial colloidal centrifugation. A manual freezing method used two freezing curves in liquid nitrogen. Sperm motility was assessed by Computer-Assisted Semen Analysis, as well as sperm capacitation-like membrane destabilisation at 30 min and 150 min after thawing using Merocyanine 540 (M540) for samples incubated at 37 °C. Spectrophotometry measured lipid peroxidation indirectly by the amount of malondialdehyde; reactive oxygen species production was also determined for sperm samples incubated for 30 minutes. The gradient centrifugation treatment improved ejaculate sperm motility and membrane destabilisation. The proportion of morphologically normal sperm was higher in the gradient than in the control. A total of 74% of spermatozoa retained normal morphology. Lipid peroxidation was lower in the colloidal centrifugation treatment. MDA was lower with gradient (16.4 ± 2.5 vs 22.3 ± 2.5 µmol/30 × 106 sperm; P < 0.05). Intracellular reactive oxygen species (ROS) and M540 positivity did not differ significantly between the treatments at either time point. The centrifugation process improved the quality parameters of frozen boar semen after thawing.

Keywords: animal science; cryopreservation; frozen-thawed sperm; oxidative stress; reproduction (search for similar items in EconPapers)
Date: 2025
References: Add references at CitEc
Citations:

Downloads: (external link)
http://cjas.agriculturejournals.cz/doi/10.17221/103/2025-CJAS.html (text/html)
http://cjas.agriculturejournals.cz/doi/10.17221/103/2025-CJAS.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjs:v:70:y:2025:i:11:id:103-2025-cjas

DOI: 10.17221/103/2025-CJAS

Access Statistics for this article

Czech Journal of Animal Science is currently edited by Bc. Michaela Polcarová

More articles in Czech Journal of Animal Science from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().