Molecular diagnosis of red rot of sugarcane in Pakistan and in vitro control of isolated pathogen using rhizobacteria

Zimen Ahmad, Muhammad Asad Farooq, Amama Fatima, Syeda Gul Nisa, Sania Tabassum, Bushra Ismat, Sundus Waseem, Umer Iqbal, Asma Akbar, Urooj Haroon, Hassan Javed Chaudhary, Tauseef Tabassum, Ahmed Z Dewidar, Abdullah Alaklabi and Muhammad Farooq Hussain Munis
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Zimen Ahmad: Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan
Muhammad Asad Farooq: Crop Disease Research Institute, National Agricultural Research Centre, Islamabad, Pakistan
Amama Fatima: Department of Plant Pathology, Faculty of Crop and Food Sciences, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan
Syeda Gul Nisa: Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan
Sania Tabassum: Department of Plant Pathology, Faculty of Crop and Food Sciences, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan
Bushra Ismat: Department of Plant Pathology, Faculty of Crop and Food Sciences, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan
Sundus Waseem: Department of Plant Pathology, Faculty of Crop and Food Sciences, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan
Umer Iqbal: Crop Disease Research Institute, National Agricultural Research Centre, Islamabad, Pakistan
Asma Akbar: Crop Disease Research Institute, National Agricultural Research Centre, Islamabad, Pakistan
Urooj Haroon: Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan
Hassan Javed Chaudhary: Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan
Tauseef Tabassum: Crop Disease Research Institute, National Agricultural Research Centre, Islamabad, Pakistan
Ahmed Z Dewidar: Prince Sultan Institute of Environmental, Water and Desert Research, King Saud University, Riyadh, Saudi Arabia
Abdullah Alaklabi: Department of Biology, Faculty of Science, University of Bisha, Bisha, Saudi Arabia
Muhammad Farooq Hussain Munis: Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan

Plant Protection Science, 2023, vol. 59, issue 2, 124-133

Abstract: Sugarcane (Saccharum officinarum L.) is a key cash crop, and its production is influenced by a variety of phytopathogens in different parts of the world. During consecutive field surveys, sugarcane stalks with red rot symptoms were observed in three provinces of Pakistan (Punjab, Sindh, and Khyber Pakhtunkhwa). Cane samples with visible symptoms were plated on Potato Dextrose Agar (PDA) media. Morphological and microscopic observations identified this pathogen as Colletotrichum falcatum. Comparative molecular study of amplified sequences of β-tubulin (Bt) and Actin (ACT) genes showed 100% similarity and ITS sequence showed 99% similarity with C. falcatum. For in vitro control of red rot, eight bacterial strains from the rhizosphere of sugarcane were isolated. These strains showed variable growth inhibition of C. falcatum in dual culture method. Among all tested strains, Bacillus amyloliquefaciens and B. altitudinis exhibited best antifungal activities. Based on these results, tested bacterial strains (B. amyloliquefaciens and B. altitudinis) can be recommended as effective biocontrol agents to manage red rot disease of sugarcane.

Keywords: sugarcane; Colletotrichum falcatum; ITS; rDNA; Bacillus (search for similar items in EconPapers)
Date: 2023
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpps:v:59:y:2023:i:2:id:45-2022-pps

DOI: 10.17221/45/2022-PPS

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