Biological activity of Paenibacillus polymyxa GT2 isolate from soil in Japan against anthracnose caused by Colletotrichum orbiculare in cucumber

Abdul Wali Haqyar, Masatoshi Ino, Naoto Kimura, Takumi Okido, Junichi Kihara and Makoto Ueno
Additional contact information
Abdul Wali Haqyar: Laboratory of Plant Pathology, Faculty of Life and Environmental Science, Shimane University, Matsue, Japan
Masatoshi Ino: The United Graduate School of Agricultural Sciences, Tottori University, Tottori, Japan
Naoto Kimura: The United Graduate School of Agricultural Sciences, Tottori University, Tottori, Japan
Takumi Okido: Department of Plant Protection, Faculty of Agriculture, Shaikh Zayed University, Khost, Afghanistan
Junichi Kihara: Department of Plant Protection, Faculty of Agriculture, Shaikh Zayed University, Khost, Afghanistan
Makoto Ueno: Department of Plant Protection, Faculty of Agriculture, Shaikh Zayed University, Khost, Afghanistan

Plant Protection Science, vol. preprint

Abstract: Cucumber anthracnose is a destructive fungal disease caused by Colletotrichum orbiculare. Common control strategies include chemical fungicides. However, this can lead to the development of pathogenic resistance. Therefore, it is necessary to identify natural compounds or microorganisms to develop new chemicals and the biological control of fungal pathogens. Isolate GT2, a bacterial isolate from soil samples collected in Shimane Prefecture, Japan, significantly inhibited in vitro mycelial growth and conidial germination of C. orbiculare, indicating a fungicidal effect against this pathogen. Furthermore, anthracnose lesion formation was significantly suppressed without phytotoxicity when cucumber leaves were pretreated with a cell culture suspension of the isolate GT2 before inoculation with C. orbiculare. Bioautography of the culture filtrate (CF) of the isolate GT2 using thin-layer chromatography showed that the compound inhibiting C. orbiculare growth had an Rf value of 0.38. The effective compound in GT2-CF was ethyl acetate insoluble and heat-stable at 121 °C and has a molecular weight larger than 1 000 Da. In conclusion, Paenibacillus polymyxa GT2 demonstrated the potential for developing a new fungicide and biological agent against anthracnose disease caused by C. orbiculare.

Keywords: biological control; disease control; fungicidal activity; inhibitory compound (search for similar items in EconPapers)
References: Add references at CitEc
Citations:

Downloads: (external link)
http://pps.agriculturejournals.cz/doi/10.17221/104/2024-PPS.html (text/html)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpps:v:preprint:id:104-2024-pps

DOI: 10.17221/104/2024-PPS

Access Statistics for this article

Plant Protection Science is currently edited by Ing. Eva Karská, (Executive Editor PPS)

More articles in Plant Protection Science from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().