Application of sonication-assisted Agrobacterium-mediated transformation in Chenopodium rubrum L

J.I. Flores Solís, P. Mlejnek, K. Studená and S. Procházka
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J.I. Flores Solís: Mendel University of Agriculture and Forestry in Brno, Czech Republic
P. Mlejnek: Mendel University of Agriculture and Forestry in Brno, Czech Republic
K. Studená: Mendel University of Agriculture and Forestry in Brno, Czech Republic
S. Procházka: Mendel University of Agriculture and Forestry in Brno, Czech Republic

Plant, Soil and Environment, 2003, vol. 49, issue 6, 255-260

Abstract: Chenopodium rubrum belongs to the plant species in which standard Agrobacterium-mediated transformation procedures remain inefficient. We demonstrate that the employment of sonication-assisted Agrobacterium-mediated transformation (SAAT) effectively enhanced transient expression of GUS gene coding for b-glucuronidase in Chenopodium rubrum. Further the results indicated that the age of seedlings is one of the limiting factors affecting the potency of Agrobacterium tumefaciens infection. Histochemical detection of b-glucuronidase activity revealed that two-days-old seedlings were much more susceptible to infection than ten-days-old ones. According to our results SAAT technology could provide an efficient tool for obtaining stable transformants when applied to two-days-old seedlings of Chenopodium rubrum.

Keywords: Agrobacterium tumefaciens; strain EHA 105; uidA; GUS; b-glucuronidase (search for similar items in EconPapers)
Date: 2003
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpse:v:49:y:2003:i:6:id:4122-pse

DOI: 10.17221/4122-PSE

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