Chromosomal rearrangements in Arabidopsis mutants revealed by repeated FISH

P. Mokroš
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P. Mokroš: Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic

Plant, Soil and Environment, 2007, vol. 53, issue 7, 325-328

Abstract: The stability of plant nuclear genome is a necessary condition for the faithful transmission of genetic information through cell lineages. When DNA damage occurs due to various impairments, cells start a number of repair processes including ligation of broken chromosomes. As a result, dicentric chromosomes can be formed. Dicentrics are easily detectable as anaphase bridges during following mitosis. Using Arabidopsis as a model plant, we developed a sensitive cytogenetic assay to identify specific chromosomal rearrangements. Here we show Arabidopsis tert-/- and atm-/- mutants and their chromosome rearrangements and fusions analysed by fluorescence in situ hybridization (FISH). The method is based on successive rounds of FISH with chromosome-specific probes and the comparison of resulting FISH images.

Keywords: Arabidopsis; FISH; chromosomal fusions; BAC probes; rDNA loci (search for similar items in EconPapers)
Date: 2007
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpse:v:53:y:2007:i:7:id:2206-pse

DOI: 10.17221/2206-PSE

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