Renaturation of telomere-binding proteins after the fractionation by SDS-polyacrylamide gel electrophoresis

G. Rotková
Additional contact information
G. Rotková: Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic

Plant, Soil and Environment, 2007, vol. 53, issue 7, 317-320

Abstract: A simple method for identification and characterization of telomere-binding proteins is described in this article. After Sodium Dodecyl Sulphate-Polyacrylamide gel electrophoresis (SDS-PAGE), proteins are eluted, renatured and used for retardation analysis with labelled oligonucleotides corresponding to human and plant of telomeric sequences. We show here that this method is efficient to recover sequence-specific DNA-binding abilities of putative telomere-binding proteins.

Keywords: method; renaturation; telomere-binding proteins; telomeres (search for similar items in EconPapers)
Date: 2007
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://pse.agriculturejournals.cz/doi/10.17221/2211-PSE.html (text/html)
http://pse.agriculturejournals.cz/doi/10.17221/2211-PSE.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpse:v:53:y:2007:i:7:id:2211-pse

DOI: 10.17221/2211-PSE

Access Statistics for this article

Plant, Soil and Environment is currently edited by Kateřina Součková

More articles in Plant, Soil and Environment from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().