Oligonucleotide microarray methodology for taxonomic and functional monitoringof microbial community composition

M. Kyselková, J. Kopecký, M. Ságová-Marečková, G.L. Grundmann and Y. Moënne-Loccoz
Additional contact information
M. Kyselková: Universitéde Lyon, F-69622, Lyon, France
J. Kopecký: Crop Research Institute, Prague, Czech Republic
M. Ságová-Marečková: Crop Research Institute, Prague, Czech Republic
G.L. Grundmann: Universitéde Lyon, F-69622, Lyon, France
Y. Moënne-Loccoz: Universitéde Lyon, F-69622, Lyon, France

Plant, Soil and Environment, 2009, vol. 55, issue 9, 379-388

Abstract: Microarray analysis is a cultivation-independent, high-throughput technology that can be used for direct and simultaneous identification of microorganisms in complex environmental samples. This review summarizes current methodologies for oligonucleotide microarrays used in microbial ecology. It deals with probe design, microarray manufacturing, sample preparation and labeling, and data handling, as well as with the key features of microarray analysis such as specificity, sensitivity and quantification potential. Microarray analysis has been validated as an effective approach to describe the composition and dynamics of taxonomic and functional microbial communities, in environments including soil, compost, sediment, air or humans. It is now part of the technical arsenal available to address key issues in microbial community ecology, ranging from biogeography to ecosystem functioning.

Keywords: Keywords: oligonucleotide microarray; taxonomic microarray; functional microarray; microbial community (search for similar items in EconPapers)
Date: 2009
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpse:v:55:y:2009:i:9:id:140-2009-pse

DOI: 10.17221/140/2009-PSE

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