The response of nitrifier, N-fixer and denitrifier gene copy numbers to the nitrification inhibitor 3,4-dimethylpyrazole phosphate

X.X. Dong, L.L. Zhang, Z.J. Wu, H.W. Zhang and P. Gong
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X.X. Dong: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, P.R. China
L.L. Zhang: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, P.R. China
Z.J. Wu: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, P.R. China
H.W. Zhang: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, P.R. China
P. Gong: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, P.R. China

Plant, Soil and Environment, 2013, vol. 59, issue 9, 398-403

Abstract: A laboratory incubation experiment was conducted to examine the effects of 3,4-dimethylpyrazole phosphate (DMPP) on the transformation of urea-N and associated microbial communities in a low-fertility brown soil. The soil was supplied with urea at 180 kg N/ha and DMPP at 0, 1.8 or 18 kg/ha, while soil that was not supplied with urea and DMPP was treated as the control. Soil mineral nitrogen (NH4+-N and NO3--N) was measured at regular intervals, and temporal variations in the population sizes of nitrogen-cycling microbes were determined using real-time PCR. Compared to the urea-alone treatment, the additional application of DMPP maintained significantly higher (P < 0.05) NH4+-N concentrations and lower NO3--N (P < 0.05) concentrations in the soil and decreased the population sizes of ammonia oxidizing and denitrifying microbes but promoted the growth of nitrogen-fixing bacteria. The results suggest that the application of DMPP is beneficial in improving the availability of fertilizer-N for plant uptake. This study is the first comprehensive investigation to examine the effects of DMPP on the functional microorganisms that are important in nitrogen-cycling processes.

Keywords: ammonia oxidizing bacteria; ammonia oxidizing archaea; nitrogen cycling; RT-PCR (search for similar items in EconPapers)
Date: 2013
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpse:v:59:y:2013:i:9:id:165-2013-pse

DOI: 10.17221/165/2013-PSE

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