Aptamers and SELEX: the technology

Tim Sampson

World Patent Information, 2003, vol. 25, issue 2, 123-129

Abstract: DNA and RNA microarrays have become an important analytical technique in the understanding and characterisation of genomes and transcriptomes. A recent development in this field of combinatorial chemistry has concentrated on using artificial DNA/RNA sequences--aptamers--as the screening ligand. In reviewing this technology, the article covers the topic, following a general introduction, under the headings: generating an aptamer library, aptamer library complexity, aspects of nucleotide chemistry, constant region primer design, and the SELEX protocol for exposing an aptamer library to the desired target.

Keywords: Aptamers; SELEX; protocol; Spiegelmers; Combinatorial; chemistry; Oligonucleotide; ligands; Screening; library; Molecular; evolution; Exponential; enrichment; Microarrays (search for similar items in EconPapers)
Date: 2003
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