Prevalence of genital mycoplasmas and co-occurrence withGardnerella vaginalisin high vaginal swabs from adult females in Bahrain: point-prevalence observational study

Shaikha Khaled AL-Enezi, Nermin Kamal Saeed, Naeema A.A. Mahmood, Mohd Shadab, Ali Al Mahmeed and Mohammad Shahid

Arab Gulf Journal of Scientific Research, 2022, vol. 41, issue 3, 376-388

Abstract: Purpose - Bacterial vaginosis (BV) is quite common and linked with serious public health issues such as premature delivery and spread of sexually transmitted infections. The study aims to identify different genital mycoplasmas (GM) in high vaginal swabs (HVS) from adult females in Bahrain. Design/methodology/approach - In total, 401 HVS were collected and cultured on MYCOFAST®RevolutioN 2 test for identification and antibiotic susceptibility. Polymerase chain reaction (PCR) was performed for detection ofMycoplasma genitalium (Mg), Mycoplasma hominis (Mh)andUreaplasmaspecies. DNA-probe based detection forGardnerella, CandidaandTrichomonaswas performed by BD Affirm Assay. Representative PCR amplicons were sequenced by Sanger sequencing. Findings - In PCR,Ureaplasmasp. was the most common GM, followed byMgandMh; the prevalence being 21.2, 5.2 and 1.5%, respectively. On the contrary, 10.7% samples showed positivity forUreaplasma urealyticum (Uu)and 1.7% for Mh in MYCOFAST®RevolutioN 2. The concordance rates between MYCOFAST®RevolutioN 2 and PCR forMhandUreaplasmasp. were 97.7 and 84%, respectively. Considering PCR as gold standard, sensitivity, specificity, positive predictive value, and negative predictive value of MYCOFAST®RevolutioN 2 were 33.3, 98.8, 28.6, 98.9 and 37.7, 96.5, 74.4, 85.2% forMhandUreaplasmasp., respectively. TheUuandMhisolates showed antibiotic-resistance ranging from 53%–58% and 71%–86%, respectively. Research limitations/implications - The prevalence ofUreaplasmasp. was high. Significant co-occurrence of GM was noticed with BV. MYCOFAST®RevolutioN 2 had lower detection-rate than PCR, so a combination is suggested for wider diagnostic coverage. Practical implications - The research reflects on status of prevalence of GM in adult females in Bahrain, and their co-occurrence with bacterial vaginosis. Diagnostic approach with combination of tests is suggested for wider coverage. The research has epidemiologic, diagnostic, and therapeutic implications. Originality/value - This is the first report from the Kingdom of Bahrain reflecting on burden of GM from this geographic location. The diagnostic efficacy of MYCOFAST® RevolutionN 2 test and polymerase chain reaction was evaluated for GM detection.

Keywords: Mycoplasma; Ureaplasma; Prevalence; Bahrain; Bacterial vaginosis; Genital mycoplasmas (search for similar items in EconPapers)
Date: 2022
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Persistent link: https://EconPapers.repec.org/RePEc:eme:agjsrp:agjsr-07-2022-0108

DOI: 10.1108/AGJSR-07-2022-0108

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