The Combination Effect of Collagen-Chitosan Hydrogel and Injectable Platelet-Rich Fibrin Fractionation on Osteoblast Migration and Proliferation

Mardha Ade Pritia, Nurul Fatimah, Vincensia Maria Karina and Kwartarini Murdiastuti
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Mardha Ade Pritia: University Gadjah Mada, Indonesia
Nurul Fatimah: Universitas Gadjah Mada, Indonesia
Vincensia Maria Karina: Universitas Gadjah Mada, Indonesia
Kwartarini Murdiastuti: Universitas Gadjah Mada, Indonesia

European Journal of Dental and Oral Health, 2023, vol. 4, issue 3, 22-27

Abstract: Injectable Platelet Rich Fibrin (i-PRF) is a popular periodontal regenerative therapy in dentistry. i-PRF centrifugation resulted in the fractionation of the yellow layer and buffy coat with a red layer. The red i-PRF contains a greater number of Growth Factor carrier cells than yellow layer, besides that liquid-PRF has a suitable consistency so it easy to combine with other biomaterials. Another biomaterial that can be combined with i-PRF is collagen-chitosan with hydrogel preparations. This study aims to determine the effect of a combination of collagen-chitosan hydrogel formulation with injection of platelet-rich fibrin fractionation on osteoblast cell migration and proliferation. This type of research was an experimental laboratory divided into 3 groups, which were given a combination of the collagen-chitosan hydrogel and i-PRF red layer, a combination of collagen-chitosan hydrogel and i-PRF yellow layer, and only a combination of collagen-chitosan hydrogel. Chitosan collagen hydrogel was prepared with a collagen/chitosan ratio of 25/75, then mixed with i-PRF fractionation with a ratio of 1:1. The cell proliferation test used CCK-8, while the cell migration test used scratch wound healing. Data were tested with One way ANOVA for cell migration and Two way ANOVA for cell proliferation, followed by a Post Hoc test. The results showed that migration of osteoblast cells after 24 hours in the combination group of chitosan collagen hydrogel and i-PRF red layer was higher than the other groups with significance (p

Keywords: Collagen; Chitosan; hydrogel; injectable platelet-rich fibrin (search for similar items in EconPapers)
Date: 2023
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Persistent link: https://EconPapers.repec.org/RePEc:epw:ejdent:v:4:y:2023:i:3:id:13264

DOI: 10.24018/ejdent.2023.4.3.264

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