Glyco-engineering Using Pneumococcal Exoglycosidases: A Pilot Study on Deglycosylation of Human Alpha-1-Acid Glycoprotein

Praveen Gautam and Neeraj Verma

International Journal of Scientific Research in Science and Technology, 2025, vol. 12, issue 1, 330-333

Abstract: Glyco-engineering is an emerging field that focuses on modifying glycan structures to enhance or alter biological functions. Enzymatic glycan remodeling has numerous applications in biopharmaceuticals, vaccine development, and glycan-based diagnostics. Pneumococcal exoglycosidases, such as neuraminidase NanA and β-galactosidase BgaA, selectively hydrolyze terminal sugars from glycoconjugates, making them potential tools for glyco-engineering. However, their ability to modify human glycoproteins in vitro remains largely unexplored. In this study, we assessed the glyco-engineering potential of pneumococcal exoglycosidases by examining their role in deglycosylating human alpha-1-acid glycoprotein (AGP), a plasma glycoprotein involved in inflammation. Our results demonstrate that NanA removes terminal sialic acid residues from AGP, enabling subsequent galactose removal by BgaA, as confirmed by SDS-PAGE and lectin blot analysis. These findings provide experimental evidence supporting the utility of pneumococcal glycosidases for enzymatic glyco-engineering.

Keywords: Glyco-engineering; sialic acid; Streptococcus pneumoniae; glycosidases; NanA; BgaA; Lectin blotting; human alpha-1-acid glycoprotein (AGP1) (search for similar items in EconPapers)
Date: 2025
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Persistent link: https://EconPapers.repec.org/RePEc:etm:ijsrst:v12:y2025:i1:id:570

DOI: 10.32628/IJSRST2512138

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