Formulation of Ointment by Integrating Melanin Pigment Isolated and Purified from Aspergillus spp. and the Comparative Analysis of Pigment’s SPF with Zinc Oxide Nanoparticles

Haider Nulwala, Prachi Matulkar and Rajitha Satish

International Journal of Scientific Research in Science and Technology, 2025, vol. 12, issue 6, 124-131

Abstract: Ultraviolet (UV) filters are essential components in the formulation of sunscreens and other photoprotective products, providing a shield against harmful UV radiation. These filters are broadly classified into organic (chemical) filters (e.g. oxybenzone) and inorganic (physical) filters (e.g. ZnO nanoparticles). This research aims at extraction, isolation and purification of a photoprotective pigment, Melanin from Aspergillus spp. Also, the research comprises the formulation of an ointment by integrating the purified pigment. Aspergillus spp. was enriched in Sabouraud's broth. After 2 weeks, the Melanin pigment was extracted and characterized by a UV-spectrophotometer. The λmax was found to be at 335 nm which confirmed presence of melanin. An ointment was formulated by mixing the pigment and molten petroleum jelly (vaseline) in 1:10 v/v. Studies were conducted with respect to the cream's stability and efficiency. Further, this study also intends to compare the sun-protecting factor (SPF) of this pigment with that of Zinc oxide nanoparticles, which are the most commonly found filters in sunscreens. Hence, ZnO nanoparticles was synthesised and characterized by UV-spectrophotometer. The peak obtained at 360 nm confirmed the synthesis of Zinc oxide nanoparticles. The SPF of the isolated and purified Melanin pigment was found to be higher.

Keywords: Ultraviolet filters; sunscreens; photoprotective pigment; melanin; Aspergillus spp.; ointment formulation; zinc oxide nanoparticles; UV - spectrophotometer; sun-protection factor (SPF); λmax (search for similar items in EconPapers)
Date: 2025
References: Add references at CitEc
Citations:

Downloads: (external link)
https://ijsrst.com/home/article/view/IJSRST25126263 Abstract page (text/html)
https://ijsrst.com/home/article/download/IJSRST25126263/IJSRST25126263 Full text (application/pdf)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:etm:ijsrst:v12:y2025:i6:id:1262

DOI: 10.32628/IJSRST25126263

Access Statistics for this article

More articles in International Journal of Scientific Research in Science and Technology from Technoscience Academy
Bibliographic data for series maintained by Pankaj Sharma ().