Extraction, Identification and Characterization by Sds-Page Method of Collagen Extracted from Rhizostoma Pulmo, a Jellyfish Found in the Black Sea Basin

Ana-Maria Pesterau, Rodica Sirbu and Emin Cadar
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Ana-Maria Pesterau: University of Medicine and and Pharmacy Carol Davila, Bucharest, Romania
Rodica Sirbu: University of Medicine and Pharmacy Carol Davila, Bucharest, Romania
Emin Cadar: “Ovidius†University of Constanta, Faculty of Pharmacy, Constanta, Romania

European Journal of Medicine and Natural Sciences, 2024, vol. 7, issue 1, 60-68

Abstract: Collagen is one of the proteins that plays an important role in many biomedical and cosmetic applications. The present study investigates collagen extracted from the jellyfish Rhizostoma pulmo, a species native to the Black Sea basin. The method of extracting collagen from the marine organism is described. The SDS-PAGE (Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis) method, an efficient technique for the separation and analysis of proteins according to molecular weight, was used to identify and characterize the protein structure of the extracted collagen. The collagen samples obtained were purified and subjected to electrophoresis, demonstrating a specific band corresponding to type I collagen, the main type of collagen found in marine organisms. The results show a protein structure similar to that of collagen isolated from other marine sources, indicating the high potential of Rhizostoma pulmo as a source of collagen for industrial applications. This research contributes to the development of sustainable methods for the valorization of marine resources from the Black Sea basin in fields such as regenerative, tissue, dermato-cosmetic, nutraceutical and even food medicine.

Keywords: collagen; marine collagen; Rhizostoma pulmo; SDS-PAGE; collagen identification (search for similar items in EconPapers)
Date: 2024
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