Ultrasensitive UPLC-MS-MS Method for the Quantitation of Etheno-DNA Adducts in Human Urine

Shiwei Cui, Haibin Li, Shaojia Wang, Xiao Jiang, Shusheng Zhang, Rongjie Zhang and Xin Sun
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Shiwei Cui: Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China
Haibin Li: Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China
Shaojia Wang: Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China
Xiao Jiang: Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China
Shusheng Zhang: Department of Chemistry, Zhengzhou University, Zhengzhou 450001, Henan province, China
Rongjie Zhang: Henan Center for Disease Control and Prevention, Zhengzhou 450016, Henan province, China
Xin Sun: Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China

IJERPH, 2014, vol. 11, issue 10, 1-13

Abstract: Etheno-DNA adducts are generated from the metabolism of exogenous carcinogens and endogenous lipid peroxidation. We and others have previously reported that 1, N 6 -ethenodeoxyadenosine (?dA) and 3, N 4 -ethenodeoxycytidine (?dC) are present in human urine and can be utilized as biomarkers of oxidative stress. In this study, we report a new ultrasensitive UPLC-ESI-MS/MS method for the analysis of ?dA and edC in human urine, capable of detecting 0.5 fmol ?dA and 0.3 fmol ?dC in 1.0 mL of human urine, respectively. For validation of the method, 20 human urine samples were analyzed, and the results revealed that the mean levels of ?dA and ?dC (SD) fmol/µmol creatinine are 5.82 ± 2.11 (range 3.0–9.5) for ?dA and 791.4 ± 328.8 (range 116.7–1264.9) for ?dC in occupational benzene-exposed workers and 2.10 ± 1.32 (range 0.6–4.7) for ?dA and 161.8 ± 200.9 (range 1.8–557.5) for ?dC in non-benzene-exposed workers, respectively. The ultrasensitive detection method is thus suitable for applications in human biomonitoring and molecular epidemiology studies.

Keywords: UPLC-MS/MS; 1, N 6 -ethenodeoxyadenosine; 3, N 4 -ethenodeoxycytidine; lipid peroxidation; human biomonitoring (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2014
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