Protective Roles of Sodium Selenite against Aflatoxin B 1 -Induced Apoptosis of Jejunum in Broilers

Xi Peng, Shengqiang Zhang, Jing Fang, Hengmin Cui, Zhicai Zuo and Junliang Deng
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Xi Peng: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China
Shengqiang Zhang: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China
Jing Fang: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China
Hengmin Cui: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China
Zhicai Zuo: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China
Junliang Deng: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China

IJERPH, 2014, vol. 11, issue 12, 1-14

Abstract: The effects of aflatoxin B 1 (AFB 1 ) exposure and sodium selenite supplementation on cell apoptosis of jejunum in broilers were studied. A total of 240 one-day-old male AA broilers were randomly assigned four dietary treatments containing 0 mg/kg of AFB 1 (control), 0.3 mg/kg AFB 1 (AFB 1 ), 0.4 mg/kg supplement Se (+ Se) and 0.3 mg/kg AFB 1 + 0.4 mg/kg supplement Se (AFB 1 + Se), respectively. Compared with the control broilers, the number of apoptotic cells, the expression of Bax and Caspase-3 mRNA were significantly increased, while the expression of Bcl-2 mRNA and the Bcl-2/Bax ratio were significantly decreased in AFB 1 broilers. The number of apoptotic cells and the expression of Caspase-3 mRNA in AFB 1 + Se broilers were significantly higher than those in the control broilers, but significantly lower than those in AFB 1 broilers. There were no significant changes in the expression of Bax mRNA between AFB 1 + Se and control broilers; the expression of Bcl-2 mRNA and the Bcl-2/Bax ratio in AFB 1 + Se broilers were significantly lower than those in the control broilers, but significantly higher than those in AFB 1 broilers. In conclusion, 0.3 mg/kg AFB 1 in the diet can increase cell apoptosis, decrease Bcl-2 mRNA expression, and increase of Bax and Caspase-3 mRNA expression in broiler’s jejunum. However, supplementation of dietary sodium selenite at the concentration of 0.4 mg/kg Se may ameliorate AFB 1 -induced apoptosis by increasing Bcl-2 mRNA expression, and decreasing Bax and Caspase-3 mRNA expression.

Keywords: aflatoxin B 1; apoptosis; TUNEL; Bcl-2; Bax; Caspase-3 (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2014
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (2)

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