Toxicity Evaluation of Pig Slurry Using Luminescent Bacteria and Zebrafish

Wenyan Chen, Qiang Cai, Yuan Zhao, Guojuan Zheng and Yuting Liang
Additional contact information
Wenyan Chen: School of Environmental and Safety Engineering, Changzhou University, No. 1 GeHu Road, Wu Jin District, Changzhou 213164, Jiangsu,China
Qiang Cai: Yangtze Delta Region Institute of Tsinghua University, No. 705, Yatai Road, Nanhu District, Jiaxing 314006, Zhejiang, China
Yuan Zhao: School of Environmental and Safety Engineering, Changzhou University, No. 1 GeHu Road, Wu Jin District, Changzhou 213164, Jiangsu,China
Guojuan Zheng: Yangtze Delta Region Institute of Tsinghua University, No. 705, Yatai Road, Nanhu District, Jiaxing 314006, Zhejiang, China
Yuting Liang: Institute of Soil Science, Chinese Academy of Science, No. 71, Beijing East Road, Nanjing 210008, Jiangsu, China

IJERPH, 2014, vol. 11, issue 7, 1-15

Abstract: Biogas slurry has become a serious pollution problem and anaerobic digestion is widely applied to pig manure treatment for environmental protection and energy recovery. To evaluate environmental risk of the emission of biogas slurry, luminescent bacteria ( Vibrio fischeri ), larvae and embryos of zebrafish ( Danio rerio ) were used to detect the acute and development toxicity of digested and post-treated slurry. Then the ability of treatment process was evaluated. The results showed that digested slurry displayed strong toxicity to both zebrafish and luminescent bacteria, while the EC 50 for luminescent bacteria and the LC 50 for larvae were only 6.81% ( v/v ) and 1.95% ( v/v ) respectively, and embryonic development was inhibited at just 1% ( v/v ). Slurry still maintained a high level of toxicity although it had been treated by membrane bioreactor (MBR), while the LC 50 of larvae was 75.23% ( v/v ) and there was a little effect on the development of embryos and V . fischeri ; the results also revealed that the zebrafish larvae are more sensitive than embryos and luminescent bacteria to pig slurry. Finally, we also found the toxicity removal rate was higher than 90% after the treatment of MBR according to toxicity tests. In conclusion, further treatment should be used in pig slurry disposal or reused of final effluent.

Keywords: pig slurry; luminescent bacteria; zebrafish; toxicity reduction (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2014
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/1660-4601/11/7/6856/pdf (application/pdf)
https://www.mdpi.com/1660-4601/11/7/6856/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jijerp:v:11:y:2014:i:7:p:6856-6870:d:37785

Access Statistics for this article

IJERPH is currently edited by Ms. Jenna Liu

More articles in IJERPH from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().