A Synthetic Thiourea-Based Tripodal Receptor that Impairs the Function of Human First Trimester Cytotrophoblast Cells

Darijana Horvat, Maryam Emami Khansari, Avijit Pramanik, Madhava R. Beeram, Thomas J. Kuehl, Md. Alamgir Hossain and Mohammad Nasir Uddin
Additional contact information
Darijana Horvat: Department of Obstetrics & Gynecology, Texas A&M Health Science Center College of Medicine/Scott & White Hospital, Temple, TX 76508, USA
Maryam Emami Khansari: Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS 39217, USA
Avijit Pramanik: Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS 39217, USA
Madhava R. Beeram: Department of Pediatrics, Texas A&M Health Science Center College of Medicine/Scott & White Hospital, Temple, TX 76508, USA
Thomas J. Kuehl: Department of Obstetrics & Gynecology, Texas A&M Health Science Center College of Medicine/Scott & White Hospital, Temple, TX 76508, USA
Md. Alamgir Hossain: Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS 39217, USA
Mohammad Nasir Uddin: Department of Obstetrics & Gynecology, Texas A&M Health Science Center College of Medicine/Scott & White Hospital, Temple, TX 76508, USA

IJERPH, 2014, vol. 11, issue 7, 1-14

Abstract: A synthetic tripodal-based thiourea receptor (PNTTU) was used to explore the receptor/ligand binding affinity using CTB cells. The human extravillous CTB cells (Sw.71) used in this study were derived from first trimester chorionic villus tissue. The cell proliferation, migration and angiogenic factors were evaluated in PNTTU-treated CTB cells. The PNTTU inhibited the CTBs proliferation and migration. The soluble fms-like tyrosine kinase-1 (sFlt-1) secretion was increased while vascular endothelial growth factor (VEGF) was decreased in the culture media of CTB cells treated with ?1 nM PNTTU. The angiotensin II receptor type 2 (AT 2 ) expression was significantly upregulated in ?1 nM PNTTU-treated CTB cells in compared to basal; however, the angiotensin II receptor, type 1 (AT 1 ) and vascular endothelial growth factor receptor 1 (VEGFR-1) expression was downregulated. The anti-proliferative and anti-angiogenic effect of this compound on CTB cells are similar to the effect of CTSs. The receptor/ligand affinity of PNTTU on CTBs provides us the clue to design a potent inhibitor to prevent the CTS-induced impairment of CTB cells.

Keywords: angiogenic; cardiotonic steroids; cell signaling; cytotrophoblast; preeclampsia; thiourea (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2014
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.mdpi.com/1660-4601/11/7/7456/pdf (application/pdf)
https://www.mdpi.com/1660-4601/11/7/7456/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jijerp:v:11:y:2014:i:7:p:7456-7469:d:38356

Access Statistics for this article

IJERPH is currently edited by Ms. Jenna Liu

More articles in IJERPH from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().