Altered Gene Transcription in Human Cells Treated with Ludox ® Silica Nanoparticles

Caterina Fede, Caterina Millino, Beniamina Pacchioni, Barbara Celegato, Chiara Compagnin, Paolo Martini, Francesco Selvestrel, Fabrizio Mancin, Lucia Celotti, Gerolamo Lanfranchi, Maddalena Mognato and Stefano Cagnin
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Caterina Fede: Department of Biology, University of Padova, Via G. Colombo 3, Padova 35121, Italy
Caterina Millino: Centro di Ricerca Interdipartimentale per le Biotecnologie Innovative—CRIBI Biotechnology Centre, University of Padova, Via U. Bassi 58/B, Padova 35121, Italy
Beniamina Pacchioni: Centro di Ricerca Interdipartimentale per le Biotecnologie Innovative—CRIBI Biotechnology Centre, University of Padova, Via U. Bassi 58/B, Padova 35121, Italy
Barbara Celegato: Centro di Ricerca Interdipartimentale per le Biotecnologie Innovative—CRIBI Biotechnology Centre, University of Padova, Via U. Bassi 58/B, Padova 35121, Italy
Chiara Compagnin: Department of Biology, University of Padova, Via G. Colombo 3, Padova 35121, Italy
Paolo Martini: Department of Biology, University of Padova, Via G. Colombo 3, Padova 35121, Italy
Francesco Selvestrel: Department of Chemical Sciences, University of Padova, via Marzolo 1, 35131 Padova, Italy
Fabrizio Mancin: Department of Chemical Sciences, University of Padova, via Marzolo 1, 35131 Padova, Italy
Lucia Celotti: Department of Biology, University of Padova, Via G. Colombo 3, Padova 35121, Italy
Gerolamo Lanfranchi: Department of Biology, University of Padova, Via G. Colombo 3, Padova 35121, Italy
Maddalena Mognato: Department of Biology, University of Padova, Via G. Colombo 3, Padova 35121, Italy
Stefano Cagnin: Department of Biology, University of Padova, Via G. Colombo 3, Padova 35121, Italy

IJERPH, 2014, vol. 11, issue 9, 1-24

Abstract: Silica (SiO 2 ) nanoparticles (NPs) have found extensive applications in industrial manufacturing, biomedical and biotechnological fields. Therefore, the increasing exposure to such ultrafine particles requires studies to characterize their potential cytotoxic effects in order to provide exhaustive information to assess the impact of nanomaterials on human health. The understanding of the biological processes involved in the development and maintenance of a variety of pathologies is improved by genome-wide approaches, and in this context, gene set analysis has emerged as a fundamental tool for the interpretation of the results. In this work we show how the use of a combination of gene-by-gene and gene set analyses can enhance the interpretation of results of in vitro treatment of A549 cells with Ludox ® colloidal amorphous silica nanoparticles. By gene-by-gene and gene set analyses, we evidenced a specific cell response in relation to NPs size and elapsed time after treatment, with the smaller NPs (SM30) having higher impact on inflammatory and apoptosis processes than the bigger ones. Apoptotic process appeared to be activated by the up-regulation of the initiator genes TNFa and IL1b and by ATM . Moreover, our analyses evidenced that cell treatment with Ludox Ò silica nanoparticles activated the matrix metalloproteinase genes MMP1 , MMP10 and MMP9 . The information derived from this study can be informative about the cytotoxicity of Ludox ® and other similar colloidal amorphous silica NPs prepared by solution processes.

Keywords: nanoparticles (NPs); cell toxicity; microarray gene expression; pathway analysis (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2014
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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