Evaluation of Immunomagnetic Separation for the Detection of Salmonella in Surface Waters by Polymerase Chain Reaction

Chao-Yu Hsu, Bing-Mu Hsu, Tien-Yu Chang, Tsui-Kang Hsu, Shu-Min Shen, Yi-Chou Chiu, Hung-Jen Wang, Wen-Tsai Ji, Cheng-Wei Fan and Jyh-Larng Chen
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Chao-Yu Hsu: Division of Urology, Department of Surgery, Tungs' Taichung MetroHarbor Hospital, Taichung 435, Taiwan
Bing-Mu Hsu: Department of Earth and Environmental Sciences, National Chung Cheng University, Chiayi 621, Taiwan
Tien-Yu Chang: Department of Radiology, Cheng Hsin General Hospital, Taipei 112, Taiwan
Tsui-Kang Hsu: Department of Ophthalmology, Cheng Hsin General Hospital, Taipei 112, Taiwan
Shu-Min Shen: Department of Earth and Environmental Sciences, National Chung Cheng University, Chiayi 621, Taiwan
Yi-Chou Chiu: General Surgery, Surgical Department, Cheng Hsin General Hospital, Taipei 112, Taiwan
Hung-Jen Wang: Department of Earth and Environmental Sciences, National Chung Cheng University, Chiayi 621, Taiwan
Wen-Tsai Ji: Department of Earth and Environmental Sciences, National Chung Cheng University, Chiayi 621, Taiwan
Cheng-Wei Fan: Department of Earth and Environmental Sciences, National Chung Cheng University, Chiayi 621, Taiwan
Jyh-Larng Chen: Department of Environmental Engineering and Health, Yuan-Pei University, Hsinchu 300, Taiwan

IJERPH, 2014, vol. 11, issue 9, 1-11

Abstract: Salmonella spp. is associated with fecal pollution and capable of surviving for long periods in aquatic environments. Instead of the traditional, time-consuming biochemical detection, polymerase chain reaction (PCR) allows rapid identification of Salmonella directly concentrated from water samples. However, prevalence of Salmonella may be underestimated because of the vulnerability of PCR to various environmental chemicals like humic acid, compounded by the fact that various DNA polymerases have different susceptibility to humic acid. Because immunomagnetic separation (IMS) theoretically could isolate Salmonella from other microbes and facilitate removal of aquatic PCR inhibitors of different sizes, this study aims to compare the efficiency of conventional PCR combined with immunomagnetic separation (IMS) for Salmonella detection within a moderately polluted watershed. In our study, the positive rate was increased from 17.6% to 47% with nearly ten-fold improvement in the detection limit. These results suggest the sensitivity of Salmonella detection could be enhanced by IMS, particularly in low quality surface waters. Due to its effects on clearance of aquatic pollutants, IMS may be suitable for most DNA polymerases for Salmonella detection.

Keywords: aquatic environment; immunomagnetic separation (IMS); Salmonella; PCR (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2014
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