Pro197Thr Substitution in Ahas Gene Causing Resistance to Pyroxsulam Herbicide in Rigid Ryegrass ( Lolium Rigidum Gaud.)

Barbara Kutasy, Zsolt Takács, Judit Kovács, Verëlindë Bogaj, Syafiq A. Razak, Géza Hegedűs, Kincső Decsi, Kinga Székvári and Eszter Virág
Additional contact information
Barbara Kutasy: Campus Keszthely, Department of Plant Physiology and Plant Ecology, Hungarian University of Agriculture and Life Sciences Georgikon, 7 Festetics Str., 8360 Keszthely, Hungary
Zsolt Takács: Department of Agricultural Mechanization, University of Pannonia, 16 Deák F. Str., 8360 Keszthely, Hungary
Judit Kovács: Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna Biocenter, Dr. Bohr-Gasse 3, 1030 Vienna, Austria
Verëlindë Bogaj: Institute of Plant Protection, University of Pannonia, 16 Deák F. Str., 8360 Keszthely, Hungary
Syafiq A. Razak: Institute of Plant Protection, University of Pannonia, 16 Deák F. Str., 8360 Keszthely, Hungary
Géza Hegedűs: Educomat Ltd., 12/A Iskola Str., 8360 Keszthely, Hungary
Kincső Decsi: Campus Keszthely, Department of Plant Physiology and Plant Ecology, Hungarian University of Agriculture and Life Sciences Georgikon, 7 Festetics Str., 8360 Keszthely, Hungary
Kinga Székvári: Festetics Doctoral School, Hungarian University of Agriculture and Life Sciences, 7 Festetics Str., 8360 Keszthely, Hungary
Eszter Virág: Educomat Ltd., 12/A Iskola Str., 8360 Keszthely, Hungary

Sustainability, 2021, vol. 13, issue 12, 1-10

Abstract: Lolium rigidum Gaud. is a cross-pollinated species characterized by high genetic diversity and it was detected as one of the most herbicide resistance-prone weeds, globally. Acetohydroxyacid synthase (AHAS) resistant populations cause significant problems in cereal production; therefore, monitoring the development of AHAS resistance is widely recommended. Using next-generation sequencing (NGS), a de novo transcriptome sequencing dataset was presented to identify the complete open reading frame (ORF) of AHAS enzyme in L. rigidum and design markers to amplify fragments consisting of all of the eight resistance-conferring amino acid mutation sites. Pro197Thr, Pro197Ala, Pro197Ser, Pro197Gln, and Trp574Leu amino acid substitutions have been observed in samples. Although the Pro197Thr amino acid substitution was already described in SU and IMI resistant populations, this is the first report to reveal that the Pro197Thr in AHAS enzyme confers a high level of resistance (ED 50 3.569) to pyroxsulam herbicide (Triazolopyrimidine).

Keywords: target-site resistance; ahas; als; Triazolopyrimidine herbicide; Lolium rigidum (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2021
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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