Application of DNA Metabarcoding for Identifying the Diet of Asian Clam ( Corbicula fluminea, Müller, 1774)

Yu-Ji Heo, Hyunbin Jo, Ji Yoon Kim, Gu-Yeon Kim, Gea-Jae Joo and Hyun-Woo Kim ()
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Yu-Ji Heo: Department of Environmental Education, Sunchon National University, Suncheon 57922, Republic of Korea
Hyunbin Jo: Department of Integrated Biological Science, Pusan National University, Busan 46241, Republic of Korea
Ji Yoon Kim: Department of Biological Science, Kunsan National University, Gunsan 54150, Republic of Korea
Gu-Yeon Kim: Department of Science Education, Kyungnam University, Changwon 51767, Republic of Korea
Gea-Jae Joo: Department of Integrated Biological Science, Pusan National University, Busan 46241, Republic of Korea
Hyun-Woo Kim: Department of Environmental Education, Sunchon National University, Suncheon 57922, Republic of Korea

Sustainability, 2022, vol. 15, issue 1, 1-14

Abstract: Corbicula has often been reported as one of the most invasive freshwater species in the world. It plays an important role in the food chains of brackish water zones in Korea, where it is predominant. However, detailed information on the Corbicula diet is still lacking. The purpose of this study was to identify the potential prey of Corbicula fluminea in the Seomjin River using a DNA metabarcoding approach, as very little is known about its feeding selectivity in natural conditions. A survey was conducted at two study sites (1 and 2) in the Seomjin River in November 2021. The two sites were selected based on increasing salinity gradient. The dominant operational taxonomic unit in the pseudofeces and gut content of C. fluminea was Microcyclops varicans (Copepoda) and Oncorhynchus mykiss (Fish), respectively. The alpha diversity at site 1 was higher than that at site 2. More diverse potential prey species were identified at the site with low salinity (i.e., site 1). The utilization of this method is strongly recommended for determining specific predator–prey relationships in complex estuarine ecosystem.

Keywords: next-generation sequencing; Corbicula fluminea; gut content; pseudofeces content; food source; Seomjin River; estuary (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2022
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