Bean Spread Enriched with Spelt as a Novel Source of Bioactive Peptides with Potential Anti-Metabolic Syndrome Properties

Paula Grdeń and Anna Jakubczyk ()
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Paula Grdeń: Department of Biochemistry and Food Chemistry, University of Life Sciences in Lublin, 20-704 Lublin, Poland
Anna Jakubczyk: Department of Biochemistry and Food Chemistry, University of Life Sciences in Lublin, 20-704 Lublin, Poland

Sustainability, 2023, vol. 15, issue 13, 1-15

Abstract: Bean spreads enriched with 10, 20, or 30% of spelt grain were analyzed in the study as a novel product with high nutraceutical potential. The spreads were hydrolyzed in vitro in gastrointestinal conditions. The highest peptide content was noted after the last step of hydrolysis in spread enriched with 10% of spelt grain (1.64 mg/mL). The fraction with molecular mass < 3.0 kDa obtained from this hydrolyzate was also characterized by the highest peptide content (1.50 mg/mL) and the highest antioxidant properties. The highest value of IC 50 against ABTS •+ was 0.078 mg/mL. The highest value of the Fe 2+ chelating activity was 0.056 mg/mL. Moreover, the trials show inhibitory activity against enzymes involved in the development of metabolic syndrome (with IC 50 values of 0.072 mg/mL for α-amylase inhibition, 0.028 mg/mL for α-glucosidase, and 0.059 mg/mL ACE—angiotensin-converting enzyme). The fraction with the highest properties was separated using Sephadex G10 and three fractions were obtained. The first and third fractions were characterized by the highest properties. These peptide fractions were identified using the LC–MS/MS technique. The following amino acid sequences were obtained from bean ( Phaseolus vulgaris ) protein: LPIESKWY, FALVAPVGSEPKA, NSILPIESKPWY, RLTDDTEDSMGRA, and KKVELEEEVDDWV, and those isolated from spelt protein had sequences FPQPQPFQ, QPQQPQQPFPQP, WPQQPQQPFPQPQQ, QSQQPQQPFPQPQQ, and QFQPQQPQQPFPQP. The study indicates that the bean spread enriched with 10 % of spelt grain may be used as a new product with special nutritional properties.

Keywords: bioactive peptides; in vitro digestion; anti-metabolic syndrome properties; bean seeds; spelt grain; metabolic syndrome (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2023
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