RNA-Pol II Transcription Elongation Factor FpRtfA Is Required for Virulence and Secondary Metabolism in Fusarium pseudograminearum

Yuxing Wu, Yajiao Wang, Sen Han, Qiusheng Li and Lingxiao Kong ()
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Yuxing Wu: Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Integrated Pest Management Center of Hebei Province, Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture, Baoding 071000, China
Yajiao Wang: Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Integrated Pest Management Center of Hebei Province, Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture, Baoding 071000, China
Sen Han: Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Integrated Pest Management Center of Hebei Province, Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture, Baoding 071000, China
Qiusheng Li: Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Integrated Pest Management Center of Hebei Province, Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture, Baoding 071000, China
Lingxiao Kong: Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Integrated Pest Management Center of Hebei Province, Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture, Baoding 071000, China

Sustainability, 2023, vol. 15, issue 14, 1-14

Abstract: The soil-borne pathogen Fusarium pseudograminearum is capable of causing a highly destructive crown disease in wheat. The purpose of this study was to characterize the biological functions, such as of virulence and secondary metabolites (SMs), of a putative RNA-Pol II transcription elongation factor, FpRtfA, in F. pseudograminearum . The current study revealed that the deletion of FpRtfA reduced radial growth compared to wild type in F. pseudograminearum . In addition, FpRtfA was found important to conidiation and response to metal ions and oxidative stress. More importantly, the virulence to the wheat stem base or head was decreased when FpRtfA was deleted. Using genome-wide gene expression profiling, FpRtfA was found to regulate several processes related to the above phenotype, such as the carbohydrate metabolic process, and the response to oxidative stress and oxidoreductase activity, especially for SMs. Further, we proved that FpRtfA exerts its regulatory effect on the virulence of pathogens by modulating the expression of the PKS gene, but not the generation of DON. In conclusion, FpRtfA has important roles in growth, asexual development, and the response to metal ions and oxidative stress. More importantly, FpRtfA is required for SMs and full virulence in F. pseudograminearum .

Keywords: Triticum aestivum; deletion mutant; conidiation; secondary metabolite; Fusarium pseudograminearum (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2023
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