New Biotechnological Production of EPA by Pythium irregulare Using Alternative Sustainable Media Obtained from Food Industry By-Products and Waste

Giovanni Luca Russo (), Antonio Luca Langellotti, Beatriz Martín-García, Vito Verardo, Raffaele Romano, Raffaele Sacchi and Paolo Masi
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Giovanni Luca Russo: Centre for Food Innovation and Development in the Food Industry, University of Naples Federico II, 80055 Portici, Italy
Antonio Luca Langellotti: Centre for Food Innovation and Development in the Food Industry, University of Naples Federico II, 80055 Portici, Italy
Beatriz Martín-García: Department of Nutrition and Food Science, Campus of Cartuja, University of Granada, 18071 Granada, Spain
Vito Verardo: Department of Nutrition and Food Science, Campus of Cartuja, University of Granada, 18071 Granada, Spain
Raffaele Romano: Unit of Food Science and Technology, Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy
Raffaele Sacchi: Unit of Food Science and Technology, Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy
Paolo Masi: Centre for Food Innovation and Development in the Food Industry, University of Naples Federico II, 80055 Portici, Italy

Sustainability, 2023, vol. 15, issue 2, 1-15

Abstract: Long-chain polyunsaturated fatty acids (LC-PUFAs) have multiple beneficial effects on human health, in particular docosahexaenoic acid (DHA, 22:6, n-3) and eicosapentaenoic acid (EPA, 20:5, n-3). A variety of microorganisms has been used for the commercial production of non-animal-source LC-PUFAs. The oomycetes of the Pythium family are promising EPA producers, and in this work, the optimization of Pythium irregulare growth using food industry by-products and wastes as cheap sources of nutrients was carried out. Sugar cane molasses (SCM), spent brewery yeast (SBY), cheese whey (CW), and expired orange juice (EFJ) were tested. A combination of SBY as a source of nitrogen and EFJ as a source of organic carbon resulted in the best outcome among the other sustainable media ingredients. The optimization of the new medium was conducted through a response surface methodology using EFJ and SBY as factors. The results show a significant positive impact of these factors on biomass productivity ( p < 0.005), with an optimized biomass yield of 14.22 g L −1 , a lipid yield of 2.23 g L −1 , and an EPA concentration of 155 mg L −1 .

Keywords: PUFA; sustainability; food waste; bioconversion; fungi; valorization (search for similar items in EconPapers)
JEL-codes: O13 Q Q0 Q2 Q3 Q5 Q56 (search for similar items in EconPapers)
Date: 2023
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