Adaptive Evolution-Assisted Riboflavin Production of Ashbya gossypii from Cane Molasses

Wenjuan Zhai, Jiantang Cheng, Yuan Li, Jianbin Li, Kai Li, Luolu Wei and Song Jiang

Mathematical Problems in Engineering, 2022, vol. 2022, 1-10

Abstract: In this study, adaptive evolution was used to improve the adaptation of Ashbya gossypii ATCC 10895 to the substrate (cane molasses) to ensure enhanced substrate utilization and increased riboflavin yield. During the adaptation process, antioxidant activity and riboflavin production ability were evaluated using shake flask fermentation to evaluate the strain response to environmental conditions. After the seventh day of fermentation, results showed that compared with the yield of the parent strain, that of A. gossypii A-24 increased by 97.5%, and the dry cell weight increased by 125%. The A-24 strain was used to further study the cofermentation process of cane molasses and glucose under different ratios. When the ratio of glucose to cane molasses was 1 : 4, the yield of riboflavin was 978 ± 5.37 mg/L, which was 3.7 times that of cane molasses monofermentation.

Date: 2022
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Persistent link: https://EconPapers.repec.org/RePEc:hin:jnlmpe:5132198

DOI: 10.1155/2022/5132198

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