Cloning of 3’ End cDNA of Ascorbate Peroxidase Gene from Fragaria × ananassa cv. Toyonaka

Yanxia Hou, Haoru Tang, Yong Zhang and Qing Chen

Journal of Agricultural Science, 2010, vol. 2, issue 2, 58

Abstract: A fragment of 3’ end cDNA of apx in Fragaria × ananassa cv. Toyonaka was cloned by rapid amplification ofcDNA 3’ end (3’RACE) PCR. By joining the sequence with the known fragment sequence which we hadsubmitted to GenBank (FJ896040), a 3’-end partial cDNA of 672bp was obtained, encoding 140 amino acids.The sequence contained the corresponding coding sequence of 424bp long including a stop codon TAA, and the3’-untranslated region(3’UTR) of 248bp including a potential polyadenylation signal (AAATAA) and poly(A) of28bp long. Homology analysis of nucleotide sequence and deduced amino acid sequence on the apx geneshowed that it shared high homology with different plants species, among which it shared the highest sequencehomology with Fragaria × ananassa cv. Yoho.

Date: 2010
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