Tunable light and drug induced depletion of target proteins

Wen Deng, Jack A. Bates, Hai Wei, Michael D. Bartoschek, Barbara Conradt and Heinrich Leonhardt ()
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Wen Deng: Ludwig-Maximilians-Universität München
Jack A. Bates: Ludwig-Maximilians-Universität München
Hai Wei: Ludwig-Maximilians-Universität München
Michael D. Bartoschek: Ludwig-Maximilians-Universität München
Barbara Conradt: Ludwig-Maximilians-Universität München
Heinrich Leonhardt: Ludwig-Maximilians-Universität München

Nature Communications, 2020, vol. 11, issue 1, 1-13

Abstract: Abstract Biological processes in development and disease are controlled by the abundance, localization and modification of cellular proteins. We have developed versatile tools based on recombinant E3 ubiquitin ligases that are controlled by light or drug induced heterodimerization for nanobody or DARPin targeted depletion of endogenous proteins in cells and organisms. We use this rapid, tunable and reversible protein depletion for functional studies of essential proteins like PCNA in DNA repair and to investigate the role of CED-3 in apoptosis during Caenorhabditis elegans development. These independent tools can be combined for spatial and temporal depletion of different sets of proteins, can help to distinguish immediate cellular responses from long-term adaptation effects and can facilitate the exploration of complex networks.

Date: 2020
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-019-14160-8

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DOI: 10.1038/s41467-019-14160-8

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