Aptamer-based optical manipulation of protein subcellular localization in cells

Sitao Xie, Yulin Du, Yu Zhang, Zhimin Wang, Dailiang Zhang, Lei He, Liping Qiu (), Jianhui Jiang () and Weihong Tan ()
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Sitao Xie: Hunan University
Yulin Du: Hunan University
Yu Zhang: Hunan University
Zhimin Wang: Hunan University
Dailiang Zhang: Hunan University
Lei He: Hunan University
Liping Qiu: Hunan University
Jianhui Jiang: Hunan University
Weihong Tan: Hunan University

Nature Communications, 2020, vol. 11, issue 1, 1-10

Abstract: Abstract Protein-dominant cellular processes cannot be fully decoded without precise manipulation of their activity and localization in living cells. Advances in optogenetics have allowed spatiotemporal control over cellular proteins with molecular specificity; however, these methods require recombinant expression of fusion proteins, possibly leading to conflicting results. Instead of modifying proteins of interest, in this work, we focus on design of a tunable recognition unit and develop an aptamer-based near-infrared (NIR) light-responsive nanoplatform for manipulating the subcellular localization of specific proteins in their native states. Our results demonstrate that this nanoplatform allows photocontrol over the cytoplasmic-nuclear shuttling behavior of the target RelA protein (a member of the NF-κβ family), enabling regulation of RelA-related signaling pathways. With a modular design, this aptamer-based nanoplatform can be readily extended for the manipulation of different proteins (e.g., lysozyme and p53), holding great potential to develop a variety of label-free protein photoregulation strategies for studying complex biological events.

Date: 2020
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DOI: 10.1038/s41467-020-15113-2

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