Size-selective molecular recognition based on a confined DNA molecular sieve using cavity-tunable framework nucleic acids

Xiaoyi Fu, Guoliang Ke, Fangqi Peng, Xue Hu, Jiaqi Li, Yuyan Shi, Gezhi Kong, Xiao-Bing Zhang () and Weihong Tan
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Xiaoyi Fu: Hunan University
Guoliang Ke: Hunan University
Fangqi Peng: Hunan University
Xue Hu: Hunan University
Jiaqi Li: Hunan University
Yuyan Shi: Hunan University
Gezhi Kong: Hunan University
Xiao-Bing Zhang: Hunan University
Weihong Tan: Hunan University

Nature Communications, 2020, vol. 11, issue 1, 1-11

Abstract: Abstract Size selectivity is an important mechanism for molecular recognition based on the size difference between targets and non-targets. However, rational design of an artificial size-selective molecular recognition system for biological targets in living cells remains challenging. Herein, we construct a DNA molecular sieve for size-selective molecular recognition to improve the biosensing selectivity in living cells. The system consists of functional nucleic acid probes (e.g., DNAzymes, aptamers and molecular beacons) encapsulated into the inner cavity of framework nucleic acid. Thus, small target molecules are able to enter the cavity for efficient molecular recognition, while large molecules are prohibited. The system not only effectively protect probes from nuclease degradation and nonspecific proteins binding, but also successfully realize size-selective discrimination between mature microRNA and precursor microRNA in living cells. Therefore, the DNA molecular sieve provides a simple, general, efficient and controllable approach for size-selective molecular recognition in biomedical studies and clinical diagnoses.

Date: 2020
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DOI: 10.1038/s41467-020-15297-7

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