Neutralization of SARS-CoV-2 spike pseudotyped virus by recombinant ACE2-Ig

Changhai Lei, Kewen Qian, Tian Li, Sheng Zhang, Wenyan Fu, Min Ding and Shi Hu ()
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Changhai Lei: Department of Biophysics, College of Basic Medical Sciences, Second Military Medical University
Kewen Qian: Department of Biophysics, College of Basic Medical Sciences, Second Military Medical University
Tian Li: Department of Biophysics, College of Basic Medical Sciences, Second Military Medical University
Sheng Zhang: Department of Critical Care Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine
Wenyan Fu: Department of Assisted Reproduction, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine
Min Ding: Pharchoice Therapeutics, Inc
Shi Hu: Department of Biophysics, College of Basic Medical Sciences, Second Military Medical University

Nature Communications, 2020, vol. 11, issue 1, 1-5

Abstract: Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in Wuhan, China, at the end of 2019, and there are currently no specific antiviral treatments or vaccines available. SARS-CoV-2 has been shown to use the same cell entry receptor as SARS-CoV, angiotensin-converting enzyme 2 (ACE2). In this report, we generate a recombinant protein by connecting the extracellular domain of human ACE2 to the Fc region of the human immunoglobulin IgG1. A fusion protein containing an ACE2 mutant with low catalytic activity is also used in this study. The fusion proteins are then characterized. Both fusion proteins have a high binding affinity for the receptor-binding domains of SARS-CoV and SARS-CoV-2 and exhibit desirable pharmacological properties in mice. Moreover, the fusion proteins neutralize virus pseudotyped with SARS-CoV or SARS-CoV-2 spike proteins in vitro. As these fusion proteins exhibit cross-reactivity against coronaviruses, they have potential applications in the diagnosis, prophylaxis, and treatment of SARS-CoV-2.

Date: 2020
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DOI: 10.1038/s41467-020-16048-4

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