Manipulating the fluorescence lifetime at the sub-cellular scale via photo-switchable barcoding

Yujie Xie, Maria C. Arno, Jonathan T. Husband, Miquel Torrent-Sucarrat and Rachel K. O’Reilly ()
Additional contact information
Yujie Xie: University of Birmingham, Edgbaston
Maria C. Arno: University of Birmingham, Edgbaston
Jonathan T. Husband: University of Birmingham, Edgbaston
Miquel Torrent-Sucarrat: Universidad del País Vasco (UPV/EHU), and Donostia International Physics Center (DIPC), Manuel Lardizabal Ibilbidea 3
Rachel K. O’Reilly: University of Birmingham, Edgbaston

Nature Communications, 2020, vol. 11, issue 1, 1-9

Abstract: Abstract Fluorescent barcoding is a pivotal technique for the investigation of the microscale world, from information storage to the monitoring of dynamic biochemical processes. Using fluorescence lifetime as the readout modality offers more reproducible and quantitative outputs compared to conventional fluorescent barcoding, being independent of sample concentration and measurement methods. However, the use of fluorescence lifetime in this area has been limited by the lack of strategies that provide spatiotemporal manipulation of the coding process. In this study, we design a two-component photo-switchable nanogel that exhibits variable fluorescence lifetime upon photoisomerization-induced energy transfer processes through light irradiation. This remotely manipulated fluorescence lifetime property could be visually mapped using fluorescence lifetime imaging microscopy (FLIM), allowing selective storage and display of information at the microscale. Most importantly, the reversibility of this system further provides a strategy for minimizing the background influence in fluorescence lifetime imaging of live cells and sub-cellular organelles.

Date: 2020
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-020-16297-3 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16297-3

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-020-16297-3

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().