AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex

Marscha Hirschi, Wang-Ting Lu, Andrew Santiago-Frangos, Royce Wilkinson, Sarah M. Golden, Alan R. Davidson (), Gabriel C. Lander () and Blake Wiedenheft ()
Additional contact information
Marscha Hirschi: The Scripps Research Institute
Wang-Ting Lu: University of Toronto
Andrew Santiago-Frangos: Montana State University
Royce Wilkinson: Montana State University
Sarah M. Golden: Montana State University
Alan R. Davidson: University of Toronto
Gabriel C. Lander: The Scripps Research Institute
Blake Wiedenheft: Montana State University

Nature Communications, 2020, vol. 11, issue 1, 1-6

Abstract: Abstract Bacteria have evolved sophisticated adaptive immune systems, called CRISPR-Cas, that provide sequence-specific protection against phage infection. In turn, phages have evolved a broad spectrum of anti-CRISPRs that suppress these immune systems. Here we report structures of anti-CRISPR protein IF9 (AcrIF9) in complex with the type I-F CRISPR RNA-guided surveillance complex (Csy). In addition to sterically blocking the hybridization of complementary dsDNA to the CRISPR RNA, our results show that AcrIF9 binding also promotes non-sequence-specific engagement with dsDNA, potentially sequestering the complex from target DNA. These findings highlight the versatility of anti-CRISPR mechanisms utilized by phages to suppress CRISPR-mediated immune systems.

Date: 2020
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-020-16512-1 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16512-1

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-020-16512-1

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().